IGI Workshop: CRISPR-Cas9 Genome Engineering & Ethics
Jennifer Doudna & Jonathan Weissman
UC Berkeley Innovative Genomics Institute (IGI) lecture series led by Jennifer Doudna and Jonathan Weissman covering the molecular mechanisms of CRISPR-Cas9, dCas9 transcriptional regulation (CRISPRi/CRISPRa), stem cell and clinical therapeutics, model organisms, and bioethics.
Syllabus
9CRISPR Biology: From Bacterial Adaptive Immunity to Genome Engineering
Jennifer Doudna, Nobel laureate and co-discoverer of CRISPR-Cas9, provides a foundational overview of CRISPR biology, tracing its development from fundamental microbial defense mechanisms to a revolutionary genome engineering technology.
Foundations of Targeted Nucleases: ZFNs, TALENs & Homologous Recombination
Dr. Dana Carroll presents foundational principles and historical context for genome editing technologies, specifically focusing on the CRISPR-Cas9 system. The talk elucidates the molecular mechanisms and applications discussed during the 2016 CRISPR Workshop.
Applications of Catalytically Inactive Cas9: CRISPRi and CRISPRa Screens
Jonathan Weissman details advanced applications of catalytically inactive Cas9 (dCas9) as a versatile tool for precise gene regulation and epigenetic engineering, beyond its traditional role in genome editing.
Precision Genome Editing in Human Pluripotent Stem Cells
Dr. Dirk Hockemeyer presents a detailed lecture on the application of CRISPR technology for precise genome editing within human stem cells, elucidating advanced molecular biology techniques.
Translating Genome Editing to the Clinic: Therapeutic Applications
Dr. Michael Holmes reviews the application of genome editing technologies, specifically CRISPR, in clinical settings, discussing the translation of these tools from laboratory research to therapeutic interventions for human disease. The lecture addresses challenges and progress in clinical trials.
High-Throughput CRISPR Mutagenesis and Phenotyping in Zebrafish
Dr. Randy Peterson from Harvard University delivers a comprehensive lecture on the methodologies and implications of applying CRISPR technology for genetic modifications in zebrafish models.
Agricultural & Livestock Genome Editing Applications
Explore the foundational science and practical implementations of CRISPR technology in livestock, covering genetic engineering for agricultural advancement.
Bioethics, Regulation & Societal Implications of Germline Editing (Part I)
This lecture, from the 2016 IGI CRISPR Workshop, analyzes the complex ethical and societal implications arising from the advent of CRISPR-Cas9 gene editing technology, focusing on its potential applications and regulatory challenges.
Scientific Governance & Ethical Oversight of Human Genome Editing (Part II)
This lecture, from the 2016 CRISPR Workshop, examines advanced ethical and societal considerations arising from CRISPR-Cas9 gene-editing technology, focusing on implications for human intervention and public policy.